Senior Associate Scientist/Principal Associate Scientist, Preclinical Research

Generation Bio

Cambridge, MA, US
  • Job Type: Full-Time
  • Function: Research Sci/Assoc/Mgr
  • Post Date: 04/29/2021
  • Website:
  • Company Address: 215 First Street, Cambridge, Cambridge, MA, MA 02142

About Generation Bio

Generation Bio is creating a revolutionary new class of genetic medicines that deliver durable, high levels of gene expression and have drug-like properties, including ability to titrate to effect and re-dose. Our unique GeneWave technology allows us to overcome the limitations of other approaches by delivering larger genes, including more sophisticated regulatory elements, and avoiding the immunogenicity which limits both the number of patients who can be treated and the ability to re-dose them over time.

Job Description

Generation Bio is an innovative genetic medicines company focused on creating a new class of non-viral gene therapy to provide durable, redosable treatments for people living with rare and prevalent diseases. The company’s non-viral platform incorporates a proprietary, high-capacity DNA construct called closed-ended DNA, or ceDNA; a cell-targeted lipid nanoparticle delivery system, or ctLNP; and an established, scalable capsid-free manufacturing process. The platform is designed to enable multi-year durability from a single dose of ceDNA and to allow titration and redosing if needed, and our scalable manufacturing process supports our mission to extend the reach of gene therapy to more people, living with more diseases, in more places around the world. Our vision is to make the ravages of genetic disease as imaginary to the next generation as polio feels to children today.

We are a thriving, collaborative, creative community of 120 people with diverse talents, beliefs, and experiences headquartered in Cambridge, Massachusetts.

Delve further. Please visit or follow @generationbio.


Generation Bio seeks an collaborative, versatile, and highly motivated Senior Associate/Principal Associate Scientist to join our Preclinical Research group. For this lab-based position, the successful candidate will be responsible for developing and implementing assays to support disease biology and advance our non viral gene therapy platform, contributing to our liver disease programs from lead optimization to candidate selection.


    Develop and execute assays to assess protein expression and distribution (e.g. immunofluorescence, Western blot, flow cytometry, qPCR) or biological effects (e.g. localization, enzyme activity, signaling pathway activation/inhibition), from tissue samples, plasma/serum, and cell culture lysates
    Conduct in vitro experiments including cell model characterization, transfection, and end point assays
    Design and troubleshoot experiments, analyze data, and interpret results
    Interface with CROs for specific study areas to coordinate research activities
    Document experiments and summarize studies in written reports and oral presentations


    Bachelor’s or Master’s in a related scientific field (e.g. Biology, Biochemistry, Molecular and Cell Biology)
    Minimum of 3-years relevant laboratory experience
    Excellent lab skills and the ability to work independently to conduct and troubleshoot experiments, as well as analyze, interpret, and present data
    Proficient in protein detection methods including immunostaining, western blot, and enzymatic assays from in vivo (tissue/plasma/serum) or in vitro (cell culture) samples
    Familiarity with cell culture manipulation techniques (e.g., transfections, cell-based assays)
    Experience with cell and tissue staining (IF, IHC, ICC) and imaging /analysis methods a plus (confocal microscopy, flow cytometry)
    Ability to multi-task to support multiple projects and work in a matrixed environment
    Prior experience with gene therapy and/or lead optimization a plus

POSITION: Full-Time, Exempt

EEOC Statement: Generation Bio believes in a diverse environment and is committed to equal employment opportunity for all its employees and qualified applicants. We do not discriminate in recruitment, hiring, training, promotion or any other employment practices for reasons of race, color, religion, gender, national origin, age, sexual orientation, marital or veteran status, disability, or any other legally protected status. Generation Bio will make reasonable accommodations for qualified individuals with known disabilities, in accordance with applicable law.

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Disclaimer: Local Candidates Only
This company does NOT accept candidates from outside recruiting firms. Agency contacts are not welcome.