Scientist, Cell Line Development

NexImmune

Gaithersburg, MD, US
  • Job Type: Full-Time
  • Function: Life Sciences R&D/Engineering
  • Post Date: 04/17/2021
  • Website: neximmune.com
  • Company Address: 22 Firstfield Road Suite 280, Gaithersburg, MD, 20878

About NexImmune

NexImmune is an emerging biopharmaceutical company advancing a new generation of immunotherapies based on our proprietary AIMâ„¢ technology.

Job Description

NexImmune is a clinical-stage biotechnology company developing a novel approach to immunotherapy designed to employ the body’s own T cells to generate a specific, potent, and durable immune response that mimics natural biology. We are seeking highly skilled and motivated individuals to join an entrepreneurial team of experienced professionals dedicated to science, patients and addressing diseases with significant unmet need. We are a company with an uncompromising commitment to shared values, a strong work ethic, and respect for individuals. We set clear expectations, empower people to succeed, and reward results. Our executive team, Board of Directors, and investors include several successful industry veterans committed to building an exceptional company capable of transforming the field of immunotherapy.

The backbone of NexImmune’s approach is its proprietary Artificial Immune Modulation (AIMTM) nanoparticle technology platform. The AIM technology enables NexImmune to construct nanoparticles that function as synthetic dendritic cells capable of directing a specific T cell-mediated immune response. By mimicking natural T cell biology, NexImmune’s T cell product candidates are designed to combine the attributes of cellular precision, potency, and persistence with reduced potential for undesired toxicities.

NexImmune’s two lead programs, NEXI-001 and NEXI-002, are in Phase 1/2 clinical trials for the treatment of relapsed AML after allogeneic stem cell transplantation and multiple myeloma refractory to at least 3 prior lines of therapy, respectively. NexImmune is also developing new AIM nanoparticle constructs and modalities for potential clinical evaluation in oncology and in disease areas outside of oncology, including autoimmune disorders and infectious disease.

For more information, visit www.neximmune.com.

Position Summary:

The Scientist I / Senior Scientist will act as a scientific contributor for the Molecular Engineering department’s support of the development of novel nanoparticles, artificial antigen presenting cell (aAPC), based upon NexImmune’s proprietary aAPC technologies. The focus of this role will revolve around cell line development, particularly using CHO and K562 cell lines, for use in protein expression and cell-base bioassays. Experience with protein expression in insect cell systems is also desirable. Expertise in molecular cloning tools and techniques are required. The candidate will support additional tissue culture activities as needed and will be responsible for coordination and transfer of cell lines to other groups for further analysis and development. Such cell lines will be pivotal for the cGMP manufacture of key signaling proteins for the preparation of aAPCs.  Standard molecular biology laboratory skills, notebook recording/maintenance, and writing of reports in acceptable scientific format(s) are required.

Essential Duties and Responsibilities:

  • Assist in the culture of mammalian cell lines
  • Assist in molecular cloning and analysis of plasmid-based protein expression vectors
  • Preparation and transfection of plasmid DNA into mammalian cells, particularly in CHO cell systems
  • Perform the selection of stable CHO cell line pools and clones utilizing published and/or commercially available systems
  • Isolation and characterization of clonal expression cell lines
  • Insect cell and baculovirus culture
  • Assist in protein purification and analysis activities
  • Order supplies and reagents and maintain inventory records
  • Write reports, protocols, and SOPs

Education and Experience:

  • Masters or PhD in Molecular Biology, Cell Biology or related field.
  • Experience working in mammalian cell culture with emphasis on the establishment of stable CHO cell lines and documentation for cGMP qualification.
  • Experience in clonal cell line isolation by limiting dilution, single cell printing, or automated clone isolation equipment
  • Familiar with writing batch record files, capturing relevant data, and presentation of data to the team and Senior Management.
  • Demonstrated proficiency in Microsoft Office, including Word, Excel, and PowerPoint.

Desired Key Competencies:

  • Excellent communication skills.
  • Self-motivated and independently minded.
  • Positive interpersonal skills with the ability to interact with individuals from a variety of levels and function.
  • Self-organizer, meticulous hands-on habits, keen attention to detail.
  • Strong quantitative and analytical skills.
  • Responsive, can-do attitude.
  • Can coordinate parallel tasks across multiple projects, demonstrating prioritization.
  • Ability to quickly adapt to a rapidly changing environment and demands.
  • Ability to interpret and effectively execute upon a variety of instructions provided in written, oral, diagram or schedule formats.

Work Environment

Frequent: Speaking; hearing; sitting; use of hands/fingers; handling or feeling objects, tools or controls; close vision; color vision; peripheral vision; ability to adjust focus

Occasional: Standing; walking indoors; reaching with hands and arms; stooping; kneeling; crouching; lifting and/or move up to 20 lbs

General: Moderate noise level, similar to typical lab and office environment with computers, printers and light traffic

Qualified, interested candidates should send a resume to: [email protected]

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Disclaimer: Local Candidates Only
This company does NOT accept candidates from outside recruiting firms. Agency contacts are not welcome.